Biosafety levels

Using the information and risk group of the agent you are working helps to determine the biosafety level required for working with the agent. Biosafety levels are the established guidelines based on the type of safety equipment required, the lab practices and procedures needed to work with the agent safely and the requirements needed for the actual facility or lab you are working in.

BSL 1    Well characterized, non-pathogenic organisms or agents  Use good laboratory practices, waste disposal, and aseptic  techniques  Open bench- no containment  Use your basic PPE!

If you work in a lab that is designated a BSL-1, the agents are well characterized, non-pathogenic organisms or agents that are not known to cause disease and present minimal potential hazard to laboratory personnel and the environment. Standard microbiological procedures are followed and work can be performed on an open bench or lab table. The laboratory is not necessarily separated from the general traffic patterns in the building but the lab should have a door and there should be controlled access to the lab during work. Appropriate PPE such as lab coats, gloves, and eye protection are worn as needed.

BSL 2    Agents of moderate hazard to personnel  or environment  Builds on BSL 1  Restricted access  Containment during certain processes  Use good laboratory practices, waste disposal, and aseptic  techniques

BSL-2 labs are suitable for work involving agents of moderate potential hazard to personnel and the environment, normally risk group 2 agents. In addition to BSL 1 requirements BSL 2 labs have more restricted access and increased containment, such as biological safety cabinets, is recommended for processes that can cause splashes or aerosols. As in all labs, good microbiological techniques, proper waste disposal and aseptic techniques are required.